Abstracts

 

7.

Stuart MK, Greenstone, MH. 1997. Immunological detection of Hymenopteran parasitism in Helicoverpa zea and Heliothis virescens. Biol Control 8:197-202 Abstract: We have developed a monoclonal antibody that recognizes the larval and adult stages of Microplitis croceipes (Cresson) (Hymenoptera: Braconidae) and Cotesia marginiventris (Cresson) (Hymenoptera: Braconidae), two principal endoparasitoids of the polyphagous pests Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) and Heliothis virescens (F.) (Lepidoptera: Noctuidae). The antibody has been incorporated into an indirect enzyme-linked immunosorbent assay that can be used to distinguish parasitized from unparasitized pests. The antibody cross-reacts with several different hymenopteran parasitoids, but not with any of the noctuid pests we have assayed. An immunoassay based on a broadly reactive antibody such as this one enables comprehensive detection of hymenopteran parasitism with a single antibody reagent.

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